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M94A2927.TXT
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1994-10-25
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Document 2927
DOCN M94A2927
TI HIV-1-gp120 causes induction of neuronal injury in vitro: prevention by
memantine.
DT 9412
AU Muller WE; Bormann J; Schroder HC; Ushijima H; Institut fur
Physiologische Chemie, Mainz, Germany.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):193 (abstract no. PB0202). Unique
Identifier : AIDSLINE ICA10/94369648
AB OBJECTIVES: Besides lymphocytes also other cells like astrocytes are
infected by HIV-1 in vitro. Primary neurons are very rarely, if at all
infected by HIV-1. MATERIALS AND METHODS: Rat cortical cell cultures
were prepared from the brains of newborn Wistar rats. After 48 h in
culture the cells were used for the experiments. Enriched GFAP-positive
human astrocytic cell cultures were prepared. RESULTS: After incubation
of rat cortical cell cultures with HIV-1 coat protein gp120 for 12 h,
cells showed apoptosis. Simultaneously, the percentage of viable cells
decreased from 94% to 33%. Memantine (1-amino-3,5-dimethyladamantane) as
well as the NMDA antagonist MK-801 prevented the effects of gp120 on
cortical cell cultures at micromolar concentrations. In human cultured
astrocytes, gp120 was ineffective with respect to DNA fragmentation and
cell toxicity. DISCUSSION AND CONCLUSION: The cytoprotective effect of
memantine in cortical cell cultures may qualify the drug for the
treatment of AIDS-related dementia. A clinical trial is in progress.
DE Animal Animals, Newborn Apoptosis/*DRUG EFFECTS Astrocytes/*DRUG
EFFECTS Cell Survival/DRUG EFFECTS Cells, Cultured Cerebral
Cortex/*CYTOLOGY Dizocilpine Maleate/PHARMACOLOGY HIV Envelope Protein
gp120/*PHARMACOLOGY *HIV-1 Memantine/*PHARMACOLOGY Neurons/*DRUG
EFFECTS Rats Rats, Wistar MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).